• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD72 Monoclonal antibody, PBS Only

CD72 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1C6

Cat no : 67424-1-PBS

Print datasheet

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

验证数据展示

  • WB analysis of Daudi using 67424-1-Ig (same clone as 67424-1-PBS)

    WB analysis of Daudi using 67424-1-Ig (same clone as 67424-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 67424-1-Ig (CD72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    IHC staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    IF Staining of human tonsillitis using 67424-1-Ig (same clone as 67424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67424-1-PBS in a different storage buffer formulation.

产品信息

67424-1-PBS targets CD72 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CD72 fusion protein Ag14405 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD72 molecule
Synonyms CD72, CD72 molecule, CD72b, Lyb 2, LYB2
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21854
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).