• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Daudi using 67424-1-Ig

    WB analysis of Daudi using 67424-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67424-1-Ig (CD72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67424-1-Ig

    IHC staining of human tonsillitis using 67424-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67424-1-Ig

    IHC staining of human tonsillitis using 67424-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67424-1-Ig (CD72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67424-1-Ig

    IF Staining of human tonsillitis using 67424-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD72 antibody (67424-1-Ig, Clone: 2C1C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CD72 Monoclonal antibody

CD72 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67424-1-Ig

产品说明书

CloneNo. 2C1C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, Ramos cells
Positive IF/ICC detected inhuman tonsillitis tissue, Ramos cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67424-1-Ig targets CD72 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原 CD72 fusion protein Ag14405 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD72 molecule
别名 CD72, CD72 molecule, CD72b, Lyb 2, LYB2
计算分子量 359 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC030227
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_2882663
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
WB protocol for CD72 antibody 67424-1-IgDownload protocol
IHC protocol for CD72 antibody 67424-1-IgDownload protocol
IF protocol for CD72 antibody 67424-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols