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验证数据展示

  • IF Staining of human tonsillitis using CL594-67424

    IF Staining of human tonsillitis using CL594-67424

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD72 antibody (CL594-67424, Clone: 2C1C6 ) at dilution of 1:100.

  • IF Staining of human tonsillitis using CL594-67424

    IF Staining of human tonsillitis using CL594-67424

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD72 antibody (CL594-67424, Clone: 2C1C6 ) at dilution of 1:100.

CoraLite®594-conjugated CD72 Monoclonal antibody

CD72 Monoclonal Antibody for IF-P
Cat No. CL594-67424

产品说明书

CloneNo. 2C1C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF-P

别名

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67424-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67424 targets CD72 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 CD72 fusion protein Ag14405 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD72 molecule
别名 CD72, CD72 molecule, CD72b, Lyb 2, LYB2
计算分子量 359 aa, 40 kDa
GenBank蛋白编号BC030227
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_2920132
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21854
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
IF protocol for CL594 CD72 antibody CL594-67424Download protocol
Standard Protocols
Click here to view our Standard Protocols