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Anti-Human CD72 Rabbit Recombinant Antibody

CD72 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241300C1

Cat no : 98090-1-RR

Print datasheet

Synonyms

LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72

验证数据展示

  • FC experiment of human PBMCs using 98090-1-RR

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1) , or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed.

  • FC experiment of human PBMCs using 98090-1-RR

    FC experiment of human PBMCs using 98090-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD72 Rabbit Recombinant Antibody (98090-1-RR, Clone:241300C1)(red) , or Isotype Control(blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).Cells were not fixed.

  • Affinity and Kinetic Characterization of 98090-1-RR

    Affinity and Kinetic Characterization of 98090-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98090-1-RR against Human CD72 were performed. The affinity constant is 10.7 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98090-1-RR targets CD72 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD72 molecule
Synonyms LYB2, Lyb 2, CD72b, CD72 molecule, B-cell differentiation antigen CD72
Calculated Molecular Weight 359 aa, 40 kDa
GenBank Accession NumberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21854
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
FC protocol for CD72 antibody 98090-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols