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CoraLite® Plus 488-conjugated CD74 Monoclonal antibody

CD74 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D8A1

Cat no : CL488-66390

Print datasheet

Synonyms

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

验证数据展示

  • FC experiment of human PBMCs using CL488-66390

    FC experiment of human PBMCs using CL488-66390

    1x10^6 human PBMCs were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CD74 Monoclonal antibody (CL488-66390, Clone: 2D8A1) or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were co-stained with 0.4 ug CoraLite® Plus 647 Anti-Human CD19 (SJ25C1) (CL647-65145, Clone: SJ25C1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Raji using CL488-66390

    IF Staining of Raji using CL488-66390

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 CD74 antibody (CL488-66390, Clone: 2D8A1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inRaji cells
Positive FC (Intra) detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 66390-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66390 targets CD74 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CD74 fusion protein Ag25503 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Synonyms Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018726
Gene Symbol CD74
Gene ID (NCBI) 972
RRIDAB_2883326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04233
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD74 antibody CL488-66390Download protocol
FC protocol for CL Plus 488 CD74 antibody CL488-66390Download protocol
Standard Protocols
Click here to view our Standard Protocols