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APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody

CD8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

UCHT4

Cat no : APC-65559

Print datasheet

Synonyms

CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2

验证数据展示

  • FC experiment of human PBMCs using APC-65559

    FC experiment of human PBMCs using APC-65559

    1x10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65559

    FC experiment of human PBMCs using APC-65559

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65559 targets CD8 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Thymocytes and Sézary T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD8a molecule
Synonyms CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01732
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
FC protocol for APC CD8 antibody APC-65559Download protocol
Standard Protocols
Click here to view our Standard Protocols