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Mouse CD80/B7-1 Recombinant antibody, PBS Only (Capture/Detector)

CD80/B7-1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241371E12

Cat no : 84137-4-PBS

Print datasheet

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

验证数据展示

  • Sandwich ELISA standard curve of MP01051-4

    Sandwich ELISA standard curve of MP01051-4

    Sandwich ELISA standard curve of MP01051-4, Mouse CD80/B7-1 Recombinant Matched Antibody Pair - PBS only. 84137-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0840. 84137-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Sample test of MP01051-4

    Sample test of MP01051-4

    The mean CD80/B7-1 concentration was determined to be 33,454.1 pg/mL in C2C12 cell extract based on a 3.2 mg/mL extract load.

  • Sample test of MP01051-4

    Sample test of MP01051-4

    Mouse spleen tissue was cultured in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD80/B7-1, and measured 10.44 pg/mL.

  • Sample test of MP01051-4

    Sample test of MP01051-4

    Serum of five mice was measured. The mouse CD80/B7-1 concentration of detected samples was determined to be 1,731.45 pg/mL with a range of 1,416.7 - 2,090.4 ng/mL.

  • Cytometric bead array standard curve of MP01051-1

    Cytometric bead array standard curve of MP01051-1

    Cytometric bead array standard curve of MP01051-1, MOUSE CD80 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84137-2-PBS. Detection antibody: 84137-4-PBS. Standard: Eg0840. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01051-2

    Cytometric bead array standard curve of MP01051-2

    Cytometric bead array standard curve of MP01051-2, MOUSE CD80 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84137-4-PBS. Detection antibody: 84137-1-PBS. Standard: Eg0840. Range: 0.313-40 ng/mL.

产品信息

84137-4-PBS targets CD80/B7-1 as part of a matched antibody pair:

MP01051-1: 84137-2-PBS capture and 84137-4-PBS detection (validated in Cytometric bead array)

MP01051-2: 84137-4-PBS capture and 84137-1-PBS detection (validated in Cytometric bead array)

MP01051-4: 84137-6-PBS capture and 84137-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD80 antigen
Synonyms Cd80, CD80 antigen, Cd28l, B7.1, B7 1
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_009855.2
Gene Symbol Cd80
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00609-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C