CoraLite® Plus 647 Anti-Rat CD80/B7-1 (3H5)

CD80/B7-1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3H5

Cat no : CL647-65217

Print datasheet

Synonyms

CD80, Cd80 molecule, B7-1, B7.1, B7 1



经过测试的应用

Positive FC detected inLPS-stimulated rat splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65217-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65217 targets CD80/B7-1 in FC applications and shows reactivity with rat samples.

Tested Applications FC Application Description
Tested Reactivity rat
Immunogen HTLV-1-transformed Lewis-S1 rat T cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name Cd80 molecule
Synonyms CD80, Cd80 molecule, B7-1, B7.1, B7 1
Calculated Molecular Weight 36 kDa
GenBank Accession NumberXM_017597885
Gene Symbol Cd80
Gene ID (NCBI) 25408
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDG3V671
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD80/B7-1 antibody CL647-65217Download protocol
Standard Protocols
Click here to view our Standard Protocols