CoraLite® Plus 647 Anti-Rat CD80/B7-1 (3H5)
CD80/B7-1 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
rat
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3H5
验证数据展示
经过测试的应用
Positive FC detected in | LPS-stimulated rat splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65217-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65217 targets CD80/B7-1 in FC applications and shows reactivity with rat samples.
Tested Applications | FC Application Description |
Tested Reactivity | rat |
Immunogen | HTLV-1-transformed Lewis-S1 rat T cell line 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | Cd80 molecule |
Synonyms | CD80, Cd80 molecule, B7-1, B7.1, B7 1 |
Calculated Molecular Weight | 36 kDa |
GenBank Accession Number | XM_017597885 |
Gene Symbol | Cd80 |
Gene ID (NCBI) | 25408 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | G3V671 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD80/B7-1 antibody CL647-65217 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |