验证数据展示
经过测试的应用
Positive FC detected in | Daudi cells |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 1 publications below |
产品信息
65083-1-Ig targets CD80 in FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
文献引用应用 | FC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Mouse / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | CD80 molecule |
别名 | B7-1, B7 1, B7, Activation B7-1 antigen, Activation B7 1 antigen |
计算分子量 | 33 kDa |
GenBank蛋白编号 | BC042665 |
基因名称 | CD80 |
Gene ID (NCBI) | 941 |
RRID | AB_2918386 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Affinity purification |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
实验方案
Product Specific Protocols | |
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FC protocol for CD80 antibody 65083-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |