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CoraLite® Plus 647 Anti-Human CD80 (B7-1) (2D10.4)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2D10.4

Cat no : CL647-65083

Print datasheet

Synonyms

Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

验证数据展示

  • FC experiment of Daudi using CL647-65083

    FC experiment of Daudi using CL647-65083

    1x10^6 Daudi cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4), or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed.

  • FC experiment of Daudi using CL647-65083

    FC experiment of Daudi using CL647-65083

    1x10^6 Daudi cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD80 (B7-1) (CL647-65083, Clone: 2D10.4), or CoraLite® Plus 647 Mouse IgG1 Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inDaudi cells, IFN-γ and LPS-stimulated THP-1-derived macrophages
For other applications, we recommend the unconjugated version of this antibody, 65083-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65083 targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD80 molecule
Synonyms Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7
Calculated Molecular Weight 33 kDa
GenBank Accession NumberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_2883670
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD80 (B7-1) antibody CL647-65083Download protocol
Standard Protocols
Click here to view our Standard Protocols