CD80/B7-1 Recombinant antibody
CD80/B7-1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241371E10
验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84137-5-RR targets CD80/B7-1 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | mouse |
| Immunogen | Fusion Protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD80 antigen |
| Synonyms | Cd80, CD80 antigen, Cd28l, B7.1, B7 1 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_009855.2 |
| Gene Symbol | Cd80 |
| Gene ID (NCBI) | 12519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q00609-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD80/B7-1 antibody 84137-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |