• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD81 (5A6)

CD81 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

5A6

Cat no : 65195-1-Ig

Print datasheet

Synonyms

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

验证数据展示

  • FC experiment of human PBMCs using 65195-1-Ig

    FC experiment of human PBMCs using 65195-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65195-1-Ig

    FC experiment of human PBMCs using 65195-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65195-1-Ig targets CD81 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human OCI-LY8 cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD81 molecule
Synonyms CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_2918465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60033
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
FC protocol for CD81 antibody 65195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols