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Cardinal Red™ Anti-Human CD81 (5A6)

CD81 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

5A6

Cat no : CR-65195

Print datasheet

Synonyms

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

验证数据展示

  • FC experiment of human PBMCs using CR-65195

    FC experiment of human PBMCs using CR-65195

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD81 (CR-65195, Clone:5A6) (red) or Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10). Cells were not fixed.

  • FC experiment of human PBMCs using CR-65195

    FC experiment of human PBMCs using CR-65195

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD81 (CR-65195, Clone:5A6) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65195 targets CD81 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human OCI-LY8 cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD81 molecule
Synonyms CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP60033
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ CD81 antibody CR-65195Download protocol
Standard Protocols
Click here to view our Standard Protocols