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验证数据展示

  • FC experiment of human PBMCs using PE-98108

    FC experiment of human PBMCs using PE-98108

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD4 Rabbit Recombinant Antibody and 5 ul PE Anti-Human CD84 Rabbit Recombinant Antibody (PE-98108, Clone: 241345A3) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using PE-98108

    FC experiment of human PBMCs using PE-98108

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD84 Rabbit Recombinant Antibody (PE-98108, Clone: 241345A3) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

PE Anti-Human CD84 Rabbit Recombinant Antibody

CD84 Recombinant Antibody for FC
Cat No. PE-98108
CloneNo. 241345A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98108 targets CD84 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD84 molecule
Synonyms Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule
Calculated Molecular Weight 345 aa, 39 kDa
GenBank Accession NumberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
RRIDAB_3674023
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UIB8
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).

实验方案

Product Specific Protocols
FC protocol for PE CD84 antibody PE-98108Download protocol
Standard Protocols
Click here to view our Standard Protocols