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Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody

CD85j / LILRB1 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/75

Cat no : 65623-1-MR

Print datasheet

Synonyms

LILRB1, ILT 2, GHI/75, FLJ37515, CD85j

验证数据展示

  • FC experiment of human PBMCs using 65623-1-MR

    FC experiment of human PBMCs using 65623-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 65623-1-MR

    FC experiment of human PBMCs using 65623-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65623-1-MR targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human Hairy Cell Leukemia Spleen Cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Synonyms LILRB1, ILT 2, GHI/75, FLJ37515, CD85j
Calculated Molecular Weight 71 kDa
GenBank Accession NumberBC015731
Gene Symbol LILRB1
Gene ID (NCBI) 10859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

实验方案

Product Specific Protocols
FC protocol for CD85j / LILRB1 antibody 65623-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols