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Anti-Mouse CD86 Rabbit Recombinant Antibody, PBS Only

CD86 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

230476B8

Cat no : 98025-1-PBS

Print datasheet

Synonyms

B7-2, B70, B7.2, B7 2, B7

验证数据展示

  • FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

    FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

    1x10^6 unstimulated or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed. This data was developed using the same antibody clone with 98025-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

    FC experiment of mouse splenocytes using 98025-1-RR (same clone as 98025-1-PBS)

    1x10^6 unstimulated (black) or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98025-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98025-1-RR

    Affinity and Kinetic Characterization of 98025-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98025-1-RR against Mouse CD86 were performed. The affinity constant is 25.4 nM.

产品信息

The immunogen of 98025-1-PBS is CD86 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 antigen
Synonyms B7-2, B70, B7.2, B7 2, B7
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_019388
Gene Symbol Cd86
Gene ID (NCBI) 12524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42082-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)