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Anti-Mouse CD86 Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

230476B8

Cat No : 98025-1-RR

Print datasheet

Synonyms

B7-2, B70, B7.2, B7 2, B7

验证数据展示

  • FC experiment of mouse splenocytes using 98025-1-RR

    FC experiment of mouse splenocytes using 98025-1-RR

    1x10^6 unstimulated or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98025-1-RR

    FC experiment of mouse splenocytes using 98025-1-RR

    1x10^6 unstimulated (black) or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98025-1-RR

    Affinity and Kinetic Characterization of 98025-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98025-1-RR against Mouse CD86 were performed. The affinity constant is 25.4 nM.

经过测试的应用

Positive FC detected inLPS treated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98025-1-RR is CD86 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 antigen
Synonyms B7-2, B70, B7.2, B7 2, B7
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_019388
Gene Symbol Cd86
Gene ID (NCBI) 12524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42082-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for CD86 antibody 98025-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols