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CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody

CD86 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230476B8

Cat no : CL647-98025

Print datasheet

Synonyms

B7-2, B70, B7.2, B7 2, B7

验证数据展示

  • FC experiment of mouse splenocytes using CL647-98025

    FC experiment of mouse splenocytes using CL647-98025

    1x10^6 unstimulated (left) or LPS treated (right) mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-98025

    FC experiment of mouse splenocytes using CL647-98025

    1x10^6 LPS treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (black). Cells were not fixed.

经过测试的应用

Positive FC detected inLPS treated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98025-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-98025 is CD86 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD86 antigen
Synonyms B7-2, B70, B7.2, B7 2, B7
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_019388
Gene Symbol Cd86
Gene ID (NCBI) 12524
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42082-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD86 antibody CL647-98025Download protocol
Standard Protocols
Click here to view our Standard Protocols