CD8b Monoclonal antibody

CD8b Monoclonal Antibody for IHC, ELISA
Cat No. 68432-1-Ig

产品说明书

CloneNo. 4B3B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, ELISA

CD8, 4B3B8, Ly3, Leu2, CD8B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68432-1-Ig targets CD8b in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 CD8b fusion protein Ag16925 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8b molecule
别名 CD8, 4B3B8, Ly3, Leu2, CD8B1
计算分子量 243 aa, 27 kDa
GenBank蛋白编号BC100913
基因名称 CD8B
Gene ID (NCBI) 926
RRIDAB_3085146
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP10966
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

实验方案

Product Specific Protocols
IHC protocol for CD8b antibody 68432-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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