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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL488-65135

    FC experiment of human peripheral blood lymphocytes using CL488-65135

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using CL488-65135

    FC experiment of human peripheral blood lymphocytes using CL488-65135

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and CoraLite® Plus 488-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

  • IF Staining of human peripheral blood mononuclear cells using CL488-65135

    IF Staining of human peripheral blood mononuclear cells using CL488-65135

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8) at dilution of 1:400.

CoraLite® Plus 488 Anti-Human CD8a (OKT8)

CD8a Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65135

Print datasheet

CloneNo. OKT8

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

IF/ICC, FC

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65135-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65135 targets CD8a in IF/ICC, FC applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Human
Immunogen Human thymocytes 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms CD8, CD8A, CD8a molecule, Leu2, MAL, p32
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2883197
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
FC protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
Standard Protocols
Click here to view our Standard Protocols