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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL488-65135

    FC experiment of human peripheral blood lymphocytes using CL488-65135

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8). Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using CL488-65135

    FC experiment of human peripheral blood lymphocytes using CL488-65135

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and CoraLite® Plus 488-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

  • IF Staining of human peripheral blood mononuclear cells using CL488-65135

    IF Staining of human peripheral blood mononuclear cells using CL488-65135

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD8a (CL488-65135, Clone: OKT8) at dilution of 1:400.

CoraLite® Plus 488 Anti-Human CD8a (OKT8)

CD8a Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65135

产品说明书

CloneNo. OKT8

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

Human

应用

IF/ICC, FC

别名

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65135-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65135 targets CD8a in IF/ICC, FC applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 Human
免疫原 Human thymocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD8a molecule
别名 CD8, CD8A, CD8a molecule, Leu2, MAL, p32
计算分子量 235 aa, 26 kDa
GenBank蛋白编号BC025715
基因名称 CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2883197
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
FC protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
Standard Protocols
Click here to view our Standard Protocols