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CD9 Recombinant antibody, PBS Only

CD9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240830A8

Cat no : 84142-1-PBS

Print datasheet

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

验证数据展示

  • WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

    WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 84142-1-RR (same clone as 84142-1-PBS)

    IF Staining of MCF-7 using 84142-1-RR (same clone as 84142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (84142-1-RR, Clone: 240830A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84142-1-RR

    Affinity and Kinetic Characterization of 84142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-1-RR against Human CD9 were performed. The affinity constant is 6.21 nM.

Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

产品信息

84142-1-PBS targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD9 molecule
Synonyms CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
Calculated Molecular Weight25 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberNM_001769.4
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21926
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).