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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

    WB analysis using 84142-1-RR (same clone as 84142-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    IHC staining of human ovary cancer using 84142-1-RR (same clone as 84142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 84142-1-RR (same clone as 84142-1-PBS)

    IF Staining of MCF-7 using 84142-1-RR (same clone as 84142-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (84142-1-RR, Clone: 240830A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84142-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84142-1-RR

    Affinity and Kinetic Characterization of 84142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-1-RR against Human CD9 were performed. The affinity constant is 6.21 nM.

CD9 Recombinant antibody, PBS Only

CD9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84142-1-PBS

产品说明书

CloneNo. 240830A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

产品信息

84142-1-PBS targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD9 molecule
别名 CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
计算分子量25 kDa
观测分子量23 kDa
GenBank蛋白编号NM_001769.4
基因名称 CD9
Gene ID (NCBI) 928
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP21926
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).