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CD9 Recombinant antibody

CD9 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240830A8

Cat no : 84142-1-RR

Print datasheet

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

验证数据展示

  • WB analysis using 84142-1-RR

    WB analysis using 84142-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84142-1-RR

    IHC staining of human ovary cancer using 84142-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary cancer using 84142-1-RR

    IHC staining of human ovary cancer using 84142-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of MCF-7 using 84142-1-RR

    IF Staining of MCF-7 using 84142-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (84142-1-RR, Clone: 240830A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84142-1-RR

    Affinity and Kinetic Characterization of 84142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-1-RR against Human CD9 were performed. The affinity constant is 6.21 nM.

经过测试的应用

Positive WB detected inA431 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84142-1-RR targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD9 molecule
Synonyms CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9
Calculated Molecular Weight25 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberNM_001769.4
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

实验方案

Product Specific Protocols
WB protocol for CD9 antibody 84142-1-RRDownload protocol
IHC protocol for CD9 antibody 84142-1-RRDownload protocol
IF protocol for CD9 antibody 84142-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols