CoraLite® Plus 488-conjugated CD90 Monoclonal antibody
CD90 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, pig, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D7D11
验证数据展示
经过测试的应用
| Positive FC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy CD90 Ready-To-Use IHC Kit. CD90 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66766-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66766 targets CD90 in FC (Intra) applications and shows reactivity with Human, pig, rat samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | Human, pig, rat |
| Immunogen | CD90 fusion protein Ag25603 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Thy-1 cell surface antigen |
| Synonyms | CD90, CDw90, FLJ33325, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1 |
| Calculated Molecular Weight | 161 aa, 18 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC065559 |
| Gene Symbol | THY1 |
| Gene ID (NCBI) | 7070 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04216 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD90 antibody CL488-66766 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
