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CD97 Monoclonal antibody

CD97 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse and More (2)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3F10C2

Cat no : 66972-1-Ig

Print datasheet

Synonyms

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

验证数据展示

  • IF Staining of Jurkat using 66972-1-Ig

    IF Staining of Jurkat using 66972-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of K-562 using 66972-1-Ig

    WB analysis of K-562 using 66972-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HL-60 cells, DC2.4 cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66972-1-Ig targets CD97 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen CD97 fusion protein Ag27991 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD97 molecule
Synonyms CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
RRIDAB_2882293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

实验方案

Product Specific Protocols
WB protocol for CD97 antibody 66972-1-IgDownload protocol
IF protocol for CD97 antibody 66972-1-IgDownload protocol
FC protocol for CD97 antibody 66972-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Int J Oral Sci

CD97 inhibits osteoclast differentiation via Rap1a/ERK pathway under compression

Authors - Wen Wang
View Article