验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HepG2 cells, HL-60 cells, DC2.4 cells |
Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
66972-1-Ig targets CD97 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | Human, Mouse |
文献引用反应性 | human, mouse |
免疫原 | CD97 fusion protein Ag27991 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | CD97 molecule |
别名 | CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1 |
计算分子量 | 722 aa, 79 kDa |
观测分子量 | 75 kDa |
GenBank蛋白编号 | BC026690 |
基因名称 | CD97 |
Gene ID (NCBI) | 976 |
RRID | AB_2882293 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P48960 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).
实验方案
Product Specific Protocols | |
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WB protocol for CD97 antibody 66972-1-Ig | Download protocol |
IF protocol for CD97 antibody 66972-1-Ig | Download protocol |
FC protocol for CD97 antibody 66972-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |