• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

    IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

    WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

CD97 Monoclonal antibody, PBS Only

CD97 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66972-1-PBS

产品说明书

CloneNo. 3F10C2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66972-1-PBS targets CD97 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原 CD97 fusion protein Ag27991 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD97 molecule
别名 CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1
计算分子量 722 aa, 79 kDa
观测分子量 75 kDa
GenBank蛋白编号BC026690
基因名称 CD97
Gene ID (NCBI) 976
RRIDAB_2882293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48960
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).