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验证数据展示

  • Sandwich ELISA standard curve of MP01403-3

    Sandwich ELISA standard curve of MP01403-3

    Sandwich ELISA standard curve of MP01403-3, Humam CD99 Recombinant Matched Antibody Pair - PBS only. 84580-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02321. 84580-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP01403-3

    Sample test of MP01403-3

    The mean CD99 concentration was determined to be 328.33 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 96.35 ng/mL in A431 cell extract based on a 1.3 mg/mL extract load.

  • Sample test of MP01403-3

    Sample test of MP01403-3

    HEK-293 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate wasremoved, assayed for human CD99, and measured 1.59 ng/mL.

  • Sample test of MP01403-3

    Sample test of MP01403-3

    Urine of six individual healthy human donors was measured. The human CD99 concentration of detected samples was determined to be 93.60 ng/mL with a range of 19.59 - 184.77 ng/mL.

  • Cytometric bead array standard curve of MP01403-1

    Cytometric bead array standard curve of MP01403-1

    Cytometric bead array standard curve of MP01403-1, CD99 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98174-1-PBS. Detection antibody: 84580-1-PBS. Standard: RP02321. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01403-2

    Cytometric bead array standard curve of MP01403-2

    Cytometric bead array standard curve of MP01403-2, CD99 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84580-2-PBS. Detection antibody: 84580-1-PBS. Standard: RP02321. Range: 0.781-100 ng/mL.

CD99 Recombinant antibody, PBS Only (Detector)

CD99 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84580-1-PBS
CloneNo. 241736H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241736H8

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84580-1-PBS targets CD99 as part of a matched antibody pair:

MP01403-1: 98174-1-PBS capture and 84580-1-PBS detection (validated in Cytometric bead array)

MP01403-2: 84580-2-PBS capture and 84580-1-PBS detection (validated in Cytometric bead array)

MP01403-3: 84580-3-PBS capture and 84580-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD99 molecule
Synonyms MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241736H8
Calculated Molecular Weight 19 kDa, 16 kDa, 17 kDa
GenBank Accession NumberBC021620
Gene Symbol CD99
Gene ID (NCBI) 4267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C