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- KD/KO Validated
CDK1-Specific Polyclonal antibody
CDK1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
201
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells |
Positive IP detected in | HeLa cells, HEK-293 cells |
Positive IHC detected in | human cervical cancer tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19532-1-AP targets CDK1-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, monkey |
Cited Reactivity | human, mouse, rat, pig, chicken, sheep |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cell division cycle 2, G1 to S and G2 to M |
Synonyms | CDC2, CDK1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1 |
Calculated Molecular Weight | 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | NM_001786 |
Gene Symbol | CDK1 |
Gene ID (NCBI) | 983 |
RRID | AB_10638617 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P06493 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
IHC protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
IF protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
IP protocol for CDK1-Specific antibody 19532-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
| ||
Cell Res Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. | ||
Nat Commun Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells | ||
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Nat Commun CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. |