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验证数据展示

  • FC experiment of MCF-7 using CL555-19532

    FC experiment of MCF-7 using CL555-19532

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human CDK1-Specific (CL555-19532) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody (CL555-30000)(Blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 555-conjugated CDK1-Specific Polyclonal antibody

CDK1-Specific Polyclonal Antibody for FC (Intra)
Cat No. CL555-19532

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

CDC2, CDK1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDK1 Ready-To-Use IHC Kit. CDK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19532-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-19532 targets CDK1-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 2, G1 to S and G2 to M
别名 CDC2, CDK1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号NM_001786
基因名称 CDK1
Gene ID (NCBI) 983
RRIDAB_2934641
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06493
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CDK1-Specific antibody CL555-19532Download protocol
Standard Protocols
Click here to view our Standard Protocols