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CDC25A Recombinant antibody, PBS Only

CDC25A Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240813F3

Cat no : 84145-2-PBS

Print datasheet

Synonyms

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

验证数据展示

  • IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDC25A Ready-To-Use IHC Kit. CDC25A primary antibody included.

产品信息

84145-2-PBS targets CDC25A in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cell division cycle 25 homolog A (S. pombe)
Synonyms M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3
Calculated Molecular Weight 59 kDa
GenBank Accession NumberNM_001789
Gene Symbol CDC25A
Gene ID (NCBI) 993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP30304
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.