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验证数据展示

  • IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25A antibody included
  • IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDC25A antibody included
  • IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

CDC25A Recombinant antibody, PBS Only

CDC25A Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84145-2-PBS

产品说明书

CloneNo. 240813F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDC25A Ready-To-Use IHC Kit. CDC25A primary antibody included.

产品信息

84145-2-PBS targets CDC25A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cell division cycle 25 homolog A (S. pombe)
别名 M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3
计算分子量 59 kDa
GenBank蛋白编号NM_001789
基因名称 CDC25A
Gene ID (NCBI) 993
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP30304
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.