验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CDC25A Ready-To-Use IHC Kit. CDC25A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84145-2-RR targets CDC25A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | cell division cycle 25 homolog A (S. pombe) |
| Synonyms | M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3 |
| Calculated Molecular Weight | 59 kDa |
| GenBank Accession Number | NM_001789 |
| Gene Symbol | CDC25A |
| Gene ID (NCBI) | 993 |
| RRID | AB_3671708 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P30304 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDC25A antibody 84145-2-RR | Download protocol |
| IF protocol for CDC25A antibody 84145-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |