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CDC26 Polyclonal antibody

CDC26 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14125-1-AP

Print datasheet

Synonyms

ANAPC12, APC12, C9orf17, CDC26

验证数据展示

  • WB analysis using 14125-1-AP

    WB analysis using 14125-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14125-1-AP (CDC26 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14125-1-AP

    IHC staining of mouse brain using 14125-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14125-1-AP (CDC26 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 14125-1-AP

    IHC staining of mouse brain using 14125-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14125-1-AP (CDC26 Antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14125-1-AP targets CDC26 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CDC26 fusion protein Ag5276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 26 homolog (S. cerevisiae)
Synonyms ANAPC12, APC12, C9orf17, CDC26
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC066300
Gene Symbol CDC26
Gene ID (NCBI) 246184
RRIDAB_2878018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC26, also named as ANAPC12, APC12 and C9orf17, is a component of the anaphase promoting complex/cyclosome (APC/C). CDC26 can be detected as 14 kDa and ~18 kDa (PMID: 15060174 /PMID: 10222126).

实验方案

Product Specific Protocols
WB protocol for CDC26 antibody 14125-1-APDownload protocol
IHC protocol for CDC26 antibody 14125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.

Authors - Kyungsoo Ha
View Article