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验证数据展示

  • FC experiment of K-562 using CL488-67239

    FC experiment of K-562 using CL488-67239

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC27; APC3 (CL488-67239, Clone:2F10G2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67239
CloneNo. 2F10G2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CDC27 Ready-To-Use IHC Kit. CDC27 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67239-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67239 targets CDC27; APC3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CDC27; APC3 fusion protein Ag25588 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 27 homolog (S. cerevisiae)
Synonyms CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC011656
Gene Symbol CDC27
Gene ID (NCBI) 996
RRIDAB_3084329
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30260
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CDC27; APC3 antibody CL488-67239Download protocol
Standard Protocols
Click here to view our Standard Protocols