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验证数据展示

  • FC experiment of K-562 using CL488-67239

    FC experiment of K-562 using CL488-67239

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC27; APC3 (CL488-67239, Clone:2F10G2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67239

产品说明书

CloneNo. 2F10G2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CDC27 Ready-To-Use IHC Kit. CDC27 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67239-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67239 targets CDC27; APC3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CDC27; APC3 fusion protein Ag25588 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 27 homolog (S. cerevisiae)
别名 CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3
计算分子量 92 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC011656
基因名称 CDC27
Gene ID (NCBI) 996
RRIDAB_3084329
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30260
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CDC27; APC3 antibody CL488-67239Download protocol
Standard Protocols
Click here to view our Standard Protocols