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验证数据展示

  • WB analysis using 28613-1-AP

    WB analysis using 28613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28613-1-AP (CDC2L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28613-1-AP

    WB analysis of HeLa using 28613-1-AP

    WB result of CDC2L1 antibody (28613-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC2L1 transfected HeLa cells.

  • IHC staining of human lung cancer using 28613-1-AP

    IHC staining of human lung cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC2L1 antibody included
  • IF Staining of HeLa using 28613-1-AP

    IF Staining of HeLa using 28613-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human lung cancer using 28613-1-AP

    IHC staining of human lung cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC2L1 antibody included
  • IHC staining of human colon cancer using 28613-1-AP

    IHC staining of human colon cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC2L1 antibody included
  • IHC staining of human colon cancer using 28613-1-AP

    IHC staining of human colon cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC2L1 antibody included
  • IF Staining of HUVEC using 28613-1-AP

    IF Staining of HUVEC using 28613-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

CDC2L1 Polyclonal antibody

CDC2L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28613-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SKOV-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CDC2L1 Ready-To-Use IHC Kit. CDC2L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28613-1-AP targets CDC2L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CDC2L1 fusion protein Ag28743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 2-like 1 (PITSLRE proteins)
Synonyms CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110
Calculated Molecular Weight 93 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberNM_001787
Gene Symbol CDC2L1
Gene ID (NCBI) 984
RRIDAB_2918181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)

实验方案

Product Specific Protocols
WB protocol for CDC2L1 antibody 28613-1-APDownload protocol
IHC protocol for CDC2L1 antibody 28613-1-APDownload protocol
IF protocol for CDC2L1 antibody 28613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols