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验证数据展示

  • IF Staining of HUVEC using CL488-28613

    IF Staining of HUVEC using CL488-28613

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CDC2L1 antibody (CL488-28613) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-28613

    FC experiment of HeLa using CL488-28613

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody (CL488-28613)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody

CDC2L1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28613

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDC2L1 Ready-To-Use IHC Kit. CDC2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28613-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28613 targets CDC2L1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 CDC2L1 fusion protein Ag28743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell division cycle 2-like 1 (PITSLRE proteins)
别名 CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110
计算分子量 93 kDa
观测分子量110 kDa
GenBank蛋白编号NM_001787
基因名称 CDC2L1
Gene ID (NCBI) 984
RRIDAB_3672831
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21127
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
FC protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
Standard Protocols
Click here to view our Standard Protocols