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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody

CDC2L1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28613

Print datasheet

Synonyms

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

验证数据展示

  • IF Staining of HUVEC using CL488-28613

    IF Staining of HUVEC using CL488-28613

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CDC2L1 antibody (CL488-28613) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-28613

    FC experiment of HeLa using CL488-28613

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody (CL488-28613)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDC2L1 Ready-To-Use IHC Kit. CDC2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28613-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28613 targets CDC2L1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CDC2L1 fusion protein Ag28743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 2-like 1 (PITSLRE proteins)
Synonyms CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110
Calculated Molecular Weight 93 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberNM_001787
Gene Symbol CDC2L1
Gene ID (NCBI) 984
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21127
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
FC protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
Standard Protocols
Click here to view our Standard Protocols