验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66295-1-Ig targets P-cadherin in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | P-cadherin fusion protein Ag4758 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cadherin 3, type 1, P-cadherin (placental) |
| Synonyms | Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin |
| Calculated Molecular Weight | 829 aa, 91 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC041846 |
| Gene Symbol | P-cadherin |
| Gene ID (NCBI) | 1001 |
| RRID | AB_2881678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P-cadherin antibody 66295-1-Ig | Download protocol |
| IHC protocol for P-cadherin antibody 66295-1-Ig | Download protocol |
| FC protocol for P-cadherin antibody 66295-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |