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P-cadherin Monoclonal antibody, PBS Only

P-cadherin Monoclonal Antibody for WB,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat No : 66295-1-PBS

Print datasheet

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

验证数据展示

  • WB analysis of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    WB analysis of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    IHC staining of human placenta using 66295-1-Ig (same clone as 66295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    FC experiment of A431 using 66295-1-Ig (same clone as 66295-1-PBS)

    1X10^6 A431 cells were stained with 0.20ug P-cadherin antibody (66295-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH. This data was developed using the same antibody clone with 66295-1-PBS in a different storage buffer formulation.

产品信息

66295-1-PBS targets P-cadherin in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,FC Application Description
Tested Reactivity human
Immunogen P-cadherin fusion protein Ag4758 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 3, type 1, P-cadherin (placental)
Synonyms Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin
Calculated Molecular Weight 829 aa, 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC041846
Gene Symbol CDH3
Gene ID (NCBI) 1001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22223
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.