验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67575 targets CDK1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | CDK1 fusion protein Ag29018 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 2, G1 to S and G2 to M |
| 别名 | CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, DKFZp686L20222, p34 protein kinase |
| 计算分子量 | 25 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC014563 |
| 基因名称 | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_3084359 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06493 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CDK1 antibody CL488-67575 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |