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CDK10 Polyclonal antibody

CDK10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30061-1-AP

Print datasheet

Synonyms

Serine/threonine-protein kinase PISSLRE, EC:2.7.11.22, Cyclin-dependent kinase 10, Cyclin dependent kinase 10

验证数据展示

  • WB analysis using 30061-1-AP

    WB analysis using 30061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30061-1-AP (CDK10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 30061-1-AP

    IF Staining of hTERT-RPE1 using 30061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CDK10 antibody (30061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive WB detected inA431 cells, K-562 cells, THP-1 cells, U2OS cells, C2C12 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CDK10 Ready-To-Use IHC Kit. CDK10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30061-1-AP targets CDK10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDK10 fusion protein Ag30365 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 10
Synonyms Serine/threonine-protein kinase PISSLRE, EC:2.7.11.22, Cyclin-dependent kinase 10, Cyclin dependent kinase 10
Calculated Molecular Weight 283 aa, 32 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC017342
Gene Symbol CDK10
Gene ID (NCBI) 8558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 10 (CDK10) is a Cdc2-related kinase that was discovered based on its homology to the Cdc2 PSTA1RE amino acid domain (PMID: 8208557). CDK10 plays a pivotal role in the regulation of fundamental cellular processes, including cell proliferation, transcription regulation, and cell cycle regulation. It partners with cyclin M to phosphorylate substrates such as ETS2 and PKN2 in order to modulate cellular growth. Initial reports have indicated that CDK10 may act as a tumor suppressor in breast cancer (PMID: 26392360). Additional studies have demonstrated tumor suppressive and oncogenic roles for CDK10 in malignancies such as hepatobiliary cancers, gastric cancer, glioma, nasopharyngeal carcinoma, and colorectal cancer(PMID: 22326270, PMID: 29512714, PMID: 23740091, PMID: 29845196)

实验方案

Product Specific Protocols
WB protocol for CDK10 antibody 30061-1-APDownload protocol
IF protocol for CDK10 antibody 30061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols