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验证数据展示

  • FC experiment of HeLa using CL647-55103

    FC experiment of HeLa using CL647-55103

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CDK3 (CL647-55103) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for FC (Intra)
Cat No. CL647-55103

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK3 Ready-To-Use IHC Kit. CDK3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55103-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-55103 targets CDK3 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase 3
别名 CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3
计算分子量 35 kDa
观测分子量 40-45 kDa
GenBank蛋白编号NM_001258
基因名称 CDK3
Gene ID (NCBI) 1018
RRIDAB_2934973
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ00526
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CDK3 antibody CL647-55103Download protocol
Standard Protocols
Click here to view our Standard Protocols