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CDK5RAP3 Polyclonal antibody
CDK5RAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human stomach tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue |
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
11007-1-AP targets CDK5RAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | mouse, bovine |
| Immunogen | CDK5RAP3 fusion protein Ag1474 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CDK5 regulatory subunit associated protein 3 |
| Synonyms | IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC009957 |
| Gene Symbol | CDK5RAP3 |
| Gene ID (NCBI) | 80279 |
| RRID | AB_2076869 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JB5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| IHC protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| IF protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| FC protocol for CDK5RAP3 antibody 11007-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.
|