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  • Featured Product
  • KD/KO Validated

CDK5RAP3 Polyclonal antibody

CDK5RAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11007-1-AP

Print datasheet

Synonyms

IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53

验证数据展示

  • WB analysis using 11007-1-AP

    WB analysis using 11007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • WB analysis of HEK-293 using 11007-1-AP

    WB analysis of HEK-293 using 11007-1-AP

    WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IF Staining of human liver using 11007-1-AP

    IF Staining of human liver using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 11007-1-AP

    IHC staining of human liver using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IHC staining of human liver using 11007-1-AP

    IHC staining of human liver using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11007-1-AP targets CDK5RAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse, bovine
Immunogen CDK5RAP3 fusion protein Ag1474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDK5 regulatory subunit associated protein 3
Synonyms IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC009957
Gene Symbol CDK5RAP3
Gene ID (NCBI) 80279
RRIDAB_2076869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

实验方案

Product Specific Protocols
WB protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IHC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IF protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
FC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,bovineWB

Int J Mol Sci

CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.

Authors - Yangyang Shen
  • KD Validated
View Article