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验证数据展示

  • WB analysis using 11007-1-AP

    WB analysis using 11007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • WB analysis of HEK-293 using 11007-1-AP

    WB analysis of HEK-293 using 11007-1-AP

    WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells.

  • IHC staining of human stomach using 11007-1-AP

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IF Staining of human liver using 11007-1-AP

    IF Staining of human liver using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 11007-1-AP

    IHC staining of human liver using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • IHC staining of human liver using 11007-1-AP

    IHC staining of human liver using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK5RAP3 antibody included
  • Featured Product
  • KD/KO验证

CDK5RAP3 Polyclonal antibody

CDK5RAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11007-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF-P, ELISA

别名

IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 600 μg/ml

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11007-1-AP targets CDK5RAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse, bovine
免疫原 CDK5RAP3 fusion protein Ag1474 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDK5 regulatory subunit associated protein 3
别名 IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53
计算分子量 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC009957
基因名称 CDK5RAP3
Gene ID (NCBI) 80279
RRIDAB_2076869
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

实验方案

Product Specific Protocols
WB protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IHC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IF protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
FC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,bovineWB

Int J Mol Sci

CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.

Authors - Yangyang Shen
  • KD Validated
View Article
humanWB

Autophagy

The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy

Authors - Jiangnan Liu
View Article