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验证数据展示

  • IF Staining of HepG2 using CL488-11007

    IF Staining of HepG2 using CL488-11007

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CDK5RAP3 antibody (CL488-11007) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CDK5RAP3 Polyclonal antibody

CDK5RAP3 Polyclonal Antibody for IF/ICC
Cat No. CL488-11007

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

C53, CDK5RAP3, HSF 27, IC53, LZAP, MST016, OK/SW cl.114, PP1553, Protein HSF 27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CDK5RAP3 Ready-To-Use IHC Kit. CDK5RAP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11007-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11007 targets CDK5RAP3 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CDK5RAP3 fusion protein Ag1474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDK5 regulatory subunit associated protein 3
Synonyms C53, CDK5RAP3, HSF 27, IC53, LZAP, MST016, OK/SW cl.114, PP1553, Protein HSF 27
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC009957
Gene Symbol CDK5RAP3
Gene ID (NCBI) 80279
RRIDAB_3672477
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JB5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDK5RAP3 antibody CL488-11007Download protocol
Standard Protocols
Click here to view our Standard Protocols