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验证数据展示

  • WB analysis using 84200-1-RR (same clone as 84200-1-PBS)

    WB analysis using 84200-1-RR (same clone as 84200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84200-1-RR (CDK6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84200-1-RR (same clone as 84200-1-PBS)

    IF Staining of Jurkat using 84200-1-RR (same clone as 84200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84200-1-RR (same clone as 84200-1-PBS)

    IF Staining of HeLa using 84200-1-RR (same clone as 84200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84200-1-RR

    Affinity and Kinetic Characterization of 84200-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84200-1-RR against Human CDK6 were performed. The affinity constant is 15.3 nM.

CDK6 Recombinant antibody, PBS Only

CDK6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84200-1-PBS

产品说明书

CloneNo. 241022F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

241022F11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.

产品信息

84200-1-PBS targets CDK6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CDK6 fusion protein Ag5600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 6
别名 241022F11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6
计算分子量 326 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC027989
基因名称 CDK6
Gene ID (NCBI) 1021
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ00534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).