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验证数据展示

  • WB analysis using 84200-1-RR

    WB analysis using 84200-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84200-1-RR (CDK6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 84200-1-RR

    IF Staining of Jurkat using 84200-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84200-1-RR

    IF Staining of HeLa using 84200-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84200-1-RR

    Affinity and Kinetic Characterization of 84200-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84200-1-RR against Human CDK6 were performed. The affinity constant is 15.3 nM.

CDK6 Recombinant antibody

CDK6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84200-1-RR

产品说明书

CloneNo. 241022F11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

241022F11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84200-1-RR targets CDK6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CDK6 fusion protein Ag5600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase 6
别名 241022F11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6
计算分子量 326 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC027989
基因名称 CDK6
Gene ID (NCBI) 1021
RRIDAB_3671756
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ00534
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

实验方案

Product Specific Protocols
WB protocol for CDK6 antibody 84200-1-RRDownload protocol
IF protocol for CDK6 antibody 84200-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols