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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated CDK6 Monoclonal antibody

CDK6 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4B9C11

Cat no : CL555-66278

Print datasheet

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

验证数据展示

  • IF Staining of HeLa using CL555-66278

    IF Staining of HeLa using CL555-66278

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 CDK6 antibody (CL555-66278, Clone: 4B9C11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-66278

    FC experiment of HeLa using CL555-66278

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CDK6 (CL555-66278, Clone:4B9C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66278-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66278 targets CDK6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CDK6 fusion protein Ag5600 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 6
Synonyms EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00534
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

实验方案

Product Specific Protocols
IF protocol for CL555 CDK6 antibody CL555-66278Download protocol
FC protocol for CL555 CDK6 antibody CL555-66278Download protocol
Standard Protocols
Click here to view our Standard Protocols