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验证数据展示

  • IF Staining of HeLa using CL555-66278

    IF Staining of HeLa using CL555-66278

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 CDK6 antibody (CL555-66278, Clone: 4B9C11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-66278

    FC experiment of HeLa using CL555-66278

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CDK6 (CL555-66278, Clone:4B9C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated CDK6 Monoclonal antibody

CDK6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66278

产品说明书

CloneNo. 4B9C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK6 Ready-To-Use IHC Kit. CDK6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66278-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66278 targets CDK6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CDK6 fusion protein Ag5600 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 6
别名 EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6
计算分子量 326 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC027989
基因名称 CDK6
Gene ID (NCBI) 1021
RRIDAB_2934670
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00534
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

实验方案

Product Specific Protocols
IF protocol for CL555 CDK6 antibody CL555-66278Download protocol
FC protocol for CL555 CDK6 antibody CL555-66278Download protocol
Standard Protocols
Click here to view our Standard Protocols