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验证数据展示

  • WB analysis using 67256-1-Ig

    WB analysis using 67256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67256-1-Ig (CDK9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67256-1-Ig

    IF Staining of HeLa using 67256-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (67256-1-Ig, Clone: 1G6B3 ) at dilution of 1:1000 and Multi-rAbCoraLite Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.

CDK9 Monoclonal antibody

CDK9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67256-1-Ig

产品说明书

CloneNo. 1G6B3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, T-47D cells, HCT 116 cells, A431 cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, ROS1728 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDK9 Ready-To-Use IHC Kit. CDK9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67256-1-Ig targets CDK9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 CDK9 fusion protein Ag29178 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 9
别名 C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK
计算分子量 372 aa, 43 kDa
观测分子量38-42 kDa, 55 kDa
GenBank蛋白编号BC001968
基因名称 CDK9
Gene ID (NCBI) 1025
RRIDAB_3085038
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

实验方案

Product Specific Protocols
WB protocol for CDK9 antibody 67256-1-IgDownload protocol
IF protocol for CDK9 antibody 67256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols