CoraLite® Plus 750-conjugated CDK9 Monoclonal antibody
CDK9 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
1G6B3
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, T-47D cells, TF-1 cells |
Planning an IHC experiment? We recommend our IHCeasy CDK9 Ready-To-Use IHC Kit. CDK9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67256-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-67256 targets CDK9 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | CDK9 fusion protein Ag29178 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase 9 |
| Synonyms | |
| Calculated Molecular Weight | 372 aa, 43 kDa |
| Observed Molecular Weight | 38-42 kDa, 55 kDa |
| GenBank Accession Number | BC001968 |
| Gene Symbol | CDK9 |
| Gene ID (NCBI) | 1025 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50750 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 CDK9 antibody CL750-67256 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |