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CDX2 Polyclonal antibody, PBS Only

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22101-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human appendicitis using 22101-1-AP (same clone as 22101-1-PBS)

    IHC staining of human appendicitis using 22101-1-AP (same clone as 22101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 22101-1-AP (same clone as 22101-1-PBS)

    IF Staining of Caco-2 using 22101-1-AP (same clone as 22101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (22101-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

产品信息

22101-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CDX2 fusion protein Ag17310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caudal type homeobox 2
Synonyms
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99626
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.