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验证数据展示

  • WB analysis using 82659-1-RR (same clone as 82659-1-PBS)

    WB analysis using 82659-1-RR (same clone as 82659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    WB analysis of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of rat colon using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of rat colon using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 82659-1-RR (same clone as 82659-1-PBS)

    IF Staining of Caco-2 using 82659-1-RR (same clone as 82659-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82659-1-RR

    Affinity and Kinetic Characterization of 82659-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

CDX2 Recombinant antibody, PBS Only

CDX2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82659-1-PBS

产品说明书

CloneNo. 3J1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

产品信息

82659-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CDX2 fusion protein Ag17310 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caudal type homeobox 2
别名 CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2
计算分子量 313 aa, 34 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC014461
基因名称 CDX2
Gene ID (NCBI) 1045
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99626
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.