验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HT-29 cells |
Positive IF/ICC detected in | HT-29 cells |
Positive FC detected in | HT-29 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68653-1-Ig targets CEACAM1/CD66a in WB, IF/ICC, FC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, FC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) |
别名 | CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1 |
计算分子量 | 526 aa, 58 kDa |
观测分子量 | 120-150 kDa |
GenBank蛋白编号 | BC014473 |
基因名称 | CEACAM1 |
Gene ID (NCBI) | 634 |
RRID | AB_3670395 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P13688 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).
实验方案
Product Specific Protocols | |
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WB protocol for CEACAM1/CD66a antibody 68653-1-Ig | Download protocol |
IF protocol for CEACAM1/CD66a antibody 68653-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |