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验证数据展示

  • WB analysis using 68653-1-Ig

    WB analysis using 68653-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68653-1-Ig (CEACAM1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HT-29 using 68653-1-Ig

    IF Staining of HT-29 using 68653-1-Ig

    Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • FC experiment of HT-29 using 68653-1-Ig

    FC experiment of HT-29 using 68653-1-Ig

    1x10^6 HT-29 cells were surface stained with 0.2 μg CEACAM1/CD66a Monoclonal antibody (68653-1-Ig, Clone:1D1H1) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3) was parallel stained as control. Cells were not fixed.

CEACAM1/CD66a Monoclonal antibody

CEACAM1/CD66a Monoclonal Antibody for WB, IF/ICC, FC, ELISA
Cat No. 68653-1-Ig

Print datasheet

CloneNo. 1D1H1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC, ELISA

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HT-29 cells
Positive IF/ICC detected inHT-29 cells
Positive FC detected inHT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68653-1-Ig targets CEACAM1/CD66a in WB, IF/ICC, FC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Synonyms CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1
Calculated Molecular Weight 526 aa, 58 kDa
Observed Molecular Weight120-150 kDa
GenBank Accession NumberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
RRIDAB_3670395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

实验方案

Product Specific Protocols
WB protocol for CEACAM1/CD66a antibody 68653-1-IgDownload protocol
IF protocol for CEACAM1/CD66a antibody 68653-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols