• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CEACAM1/CD66a Recombinant antibody

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240497B4

Cat no : 83524-3-RR

Print datasheet

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

验证数据展示

  • WB analysis using 83524-3-RR

    WB analysis using 83524-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83524-3-RR

    IHC staining of human ovary cancer using 83524-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83524-3-RR

    IHC staining of human ovary cancer using 83524-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83524-3-RR

    Affinity and Kinetic Characterization of 83524-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83524-3-RR against Human CEACAM1 were performed. The affinity constant is 3.58 nM.

经过测试的应用

Positive WB detected inHepG2 cells, HT-29 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83524-3-RR is CEACAM1/CD66a Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Synonyms CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1
Calculated Molecular Weight 526 aa, 58 kDa
Observed Molecular Weight135-150 kDa
GenBank Accession NumberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

实验方案

Product Specific Protocols
WB protocol for CEACAM1/CD66a antibody 83524-3-RRDownload protocol
IHC protocol for CEACAM1/CD66a antibody 83524-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols